IDENTIFICATION OF HIV-INFECTED SERONEGATIVE INDIVIDUALS BY A DIRECT DIAGNOSTIC TEST BASED ON HYBRIDISATION TO AMPLIFIED VIRAL DNA
Identifieur interne : 001575 ( Main/Exploration ); précédent : 001574; suivant : 001576IDENTIFICATION OF HIV-INFECTED SERONEGATIVE INDIVIDUALS BY A DIRECT DIAGNOSTIC TEST BASED ON HYBRIDISATION TO AMPLIFIED VIRAL DNA
Auteurs : Michel Loche [Suisse] ; Bernard Mach [Suisse]Source :
- The Lancet [ 0140-6736 ] ; 1988.
English descriptors
- KwdEn :
- Aids virus, Amplification, Amplification cycles, Amplification procedure, Amplification reaction, Antibody response, Blood samples, Breastfed children, Clin nutr, Deposition, Diagnostic procedure, Diagnostic test, Direct detection, Enzymatic amplification, Ethanol precipitation, Fibrin, Fibrin deposition, Human immunodeficiency virus, Hybridisation, Hybridisation test, Large scale, Lymphocyte, Macrophage, Monoclonal antibodies, Oligo probe, Plasmid clone, Primer, Serological diagnosis, Serological response, Seronegative, Seronegative partners, Seropositive, Seropositive individuals, Seropositive subject, Seropositive subjects, Several months, Sexual partners, Sodium chloride, Sodium dodecylsulphate, Specific segment, Viral, Viral genome.
- Teeft :
- Aids virus, Amplification, Amplification cycles, Amplification procedure, Amplification reaction, Antibody response, Blood samples, Breastfed children, Clin nutr, Deposition, Diagnostic procedure, Diagnostic test, Direct detection, Enzymatic amplification, Ethanol precipitation, Fibrin, Fibrin deposition, Human immunodeficiency virus, Hybridisation, Hybridisation test, Large scale, Lymphocyte, Macrophage, Monoclonal antibodies, Oligo probe, Plasmid clone, Primer, Serological diagnosis, Serological response, Seronegative, Seronegative partners, Seropositive, Seropositive individuals, Seropositive subject, Seropositive subjects, Several months, Sexual partners, Sodium chloride, Sodium dodecylsulphate, Specific segment, Viral, Viral genome.
Abstract
There is a need for direct detection of the virus in people infected with human immunodeficiency virus (HIV), independently of a serological response. In this study, after enzymic amplification of a specific segment of the HIV genome, a simple slot-blot hybridisation procedure allowed unequivocal identification of HIV DNA in all seropositive subjects tested. More importantly, the hybridisation test allowed the detection of HIV DNA in several seronegative subjects from very high risk groups. This new direct approach towards the diagnosis of HIV infection, which can easily be carried out on a large scale, is therefore capable of identifying HIV-infected individuals before the development of antibodies.
Url:
DOI: 10.1016/S0140-6736(88)90412-6
Affiliations:
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Le document en format XML
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partners</term><term>Seropositive</term><term>Seropositive individuals</term><term>Seropositive subject</term><term>Seropositive subjects</term><term>Several months</term><term>Sexual partners</term><term>Sodium chloride</term><term>Sodium dodecylsulphate</term><term>Specific segment</term><term>Viral</term><term>Viral genome</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Aids virus</term><term>Amplification</term><term>Amplification cycles</term><term>Amplification procedure</term><term>Amplification reaction</term><term>Antibody response</term><term>Blood samples</term><term>Breastfed children</term><term>Clin nutr</term><term>Deposition</term><term>Diagnostic procedure</term><term>Diagnostic test</term><term>Direct detection</term><term>Enzymatic amplification</term><term>Ethanol precipitation</term><term>Fibrin</term><term>Fibrin deposition</term><term>Human immunodeficiency virus</term><term>Hybridisation</term><term>Hybridisation test</term><term>Large scale</term><term>Lymphocyte</term><term>Macrophage</term><term>Monoclonal antibodies</term><term>Oligo probe</term><term>Plasmid clone</term><term>Primer</term><term>Serological diagnosis</term><term>Serological response</term><term>Seronegative</term><term>Seronegative partners</term><term>Seropositive</term><term>Seropositive individuals</term><term>Seropositive subject</term><term>Seropositive subjects</term><term>Several months</term><term>Sexual partners</term><term>Sodium chloride</term><term>Sodium dodecylsulphate</term><term>Specific segment</term><term>Viral</term><term>Viral genome</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">There is a need for direct detection of the virus in people infected with human immunodeficiency virus (HIV), independently of a serological response. In this study, after enzymic amplification of a specific segment of the HIV genome, a simple slot-blot hybridisation procedure allowed unequivocal identification of HIV DNA in all seropositive subjects tested. More importantly, the hybridisation test allowed the detection of HIV DNA in several seronegative subjects from very high risk groups. 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